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Study the Force Response of Filamin A by Improved Magnetic Tweezers
2012-06-29  【 】【打印】【关闭
Institute of Theoretical Physics
Chinese Academy of Sciences
学术报告
Title
题目
Study the Force Response of Filamin A by Improved Magnetic Tweezers
Speaker
报告人
陈虎博士
Mechanobiology Institute, National University of Singapore
Date
日期
2012-06-29 PM 16:00 Friday
Venue
地点
Conference Hall 322, ITP/理论物理所322报告厅
Abstract
摘要

Filamin A, an actin cross-linking protein, consists of two subunits that dimerize through C-terminal self-association domain. Each subunit contains an N-terminal spectrin-related actin-binding domain followed by 24 immunoglobulin-like (Ig) repeats. Two flexible hinges separate the 24 Ig repeats into rod 1 (repeats 1-15), rod 2 (repeats 16-23), and self-association domain 24. Filamin A not only supports the tension of actin network but also interacts with many transmembrane and signaling proteins mostly in the rod 2 segment. By using high force magnetic tweezers, the force response of Filamin A was studied. We found that rod 2 segment is susceptible to ~10 pN force, while the rod 1 segment can withstand significantly higher forces. Physiological-relevant-force-induced conformation change in rod 2 segment is from the disruption of domain pair interaction, which supports the mechano-sensing function of Filamin A.

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